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Interaction between SigE and ChlH in vitro: GST-pulldown analysis. Various amounts of GST or GST-ChlH were bound to glutathione-sepharose 4B beads and mixed with His-SigE (1.0 μg), after which bead-bound proteins were subjected to immunoblotting with antiserum to SigE. Data represent mean ± SD of values from 3 independent experiments. The level is calibrated relative to the pull-down value at 6 μg GST-ChlH, which is set at 100%.
