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Immunoprecipitation analysis. Proteins from GT Synechocystis 6803 cells grown in modified BG-11 (A750 = ≈2.0) under light or dark conditions (1 h) were mixed with 50 μL of rabbit preimmune antiserum, rabbit antiserum against ChlH, or rat antiserum against SigE. Proteins from the collected cells were extracted in PBS containing 1 mM MgCl2. Precipitated SigE or ChlH proteins were detected by immunoblotting with rabbit or rat SigE or rabbit ChlH antiserum.
