Fig. 1.

Activation of PKC enhances the amplitude and frequency of AMPA mEPSCs. (A) Representative recordings of mEPSCs in an embryonic zebrafish Mauthner-cell before and after application of PMA (100 nM) and the PKC inhibitor BIS 1 (500 nM). (B) PMA increased the mean mEPSC amplitude and frequency (n = 7, P < 0.001). Intracellular application of Bisindolymaleimide I (BIS I, 500 nM; n = 6), before PMA application, blocked the increase in amplitude (n = 6, P = 0.676), but had no effect on the frequency. (C) Inclusion of the Ca²⁺-chelating agent, BAPTA (5 mM), in the patch pipette blocked the PMA-induced increase in amplitude (n = 6, P < 0.783), but had no effect on the mEPSC frequency. ***, significantly different, P < 0.001.