Figure 6. Effect of PKCε ASO on ethanol-induced steatosis.
Oil red O staining was performed as described in Materials and Methods. Representative photomicrographs (200×magnification) of saline-treated wild-type mice receiving maltose-dextrin (upper right panel) and ethanol (upper left panel), as well as PKCε ASO treated-mice receiving ethanol (lower left panel) are shown. Quantitation of triglycerides (lower right panel) was performed via a colorimetric assay as described in Materials and Methods. Data represent means±SEM (n=4–6) and are normalized to mg protein. *, p<0.05 compared to the absence of ethanol; #, p<0.05 compared with wild-type or saline-treated mice exposed to ethanol.