Fig. 4.

Synapse formation induced by lithium requires translation and neurotransmission. A, processed images acquired before and after 4-h treatment with 5 mM lithium in the absence (control) and presence of 10 μM cycloheximide display labeled PSDs superimposed on DsRed2 fluorescence. Scale bar, 10 μm. B, bar graph shows change in the number of PSDs after 4-h treatment with vehicle (control, □) or 5 mM lithium (▪) in the absence (untreated) or presence of 10 μM MK801, 10 μM CNQX, or 10 μM cycloheximide (CHX) as indicated. Data are presented as mean ± S.E.M. ^*, p < 0.01 compared with untreated control; #, p < 0.01 compared with lithium-induced response in the absence of drug (untreated), ANOVA with Bonferroni's multiple comparison test.