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. 2009 Feb 13;75(5):1084–1095. doi: 10.1124/mol.108.054353

Fig. 4.

Fig. 4.

Comparison of nAChR inhibition by the open channel blocker QX-222 (100 μM, A and B) and by azietomidate (10 μM, C and D). T. californica nAChRs were exposed simultaneously to 300 μM ACh and inhibitor according to four different protocols that are numbered on the traces: 1) 800 ms of ACh, before return to buffer; 2) 800 ms of ACh + inhibitor, before return to buffer; 3) 30 ms of ACh + QX-222 (A) or 100 ms of ACh + azietomidate (C), before return to buffer; or 4) 30 ms of ACh + QX-222 (B) or ACh + azietomidate (D), before return to ACh alone for 170 ms. In each panel, the bar above the current traces indicates the duration of exposure to ACh and QX-222 or azietomidate before switching the patch to a stream of buffer (A and C) or ACh (B and D). A and B are all from a single patch, as are those from C and D. Each trace is an average of four sweeps, with a 20- to 120-s buffer wash between each trial.