Fig. 9.

Effect of cocaine and SoRI-9804 on d-amphetamine induced DAT-mediated efflux of [³H]MPP⁺. As described under Materials and Methods, synaptosomes were preloaded with [³H]MPP⁺ for 60 min. d-Amphetamine (100 nM) was added in the absence and presence of various concentrations of cocaine (A) or SoRI-9804 (B), and samples were filtered at various times up to 60 min later. Each value is the mean ± S.D. The data of three independent experiments were pooled and fit to one- and two-component dissociation experiments using MLAB-PC. The best-fit estimates of the kinetic parameters are reported in Table 4 for cocaine and in Table 5 for SoRI-9804.