TABLE 5.

Effect of SoRI-9804 on d-amphetamine-induced [³H]MPP⁺ efflux [³H]MPP⁺ efflux experiments were conducted as described under Materials and Methods to determine the effect of SoRI-9804 on d-amphetamine-induced (100 nM) [³H]MPP⁺ efflux. The data of three independent experiments were pooled and fit to one- and two-component dissociation experiments using MLAB-PC. The parameter values are ± S.D. In all cases, the two-component model fit significantly better than the one-component model (F-test, P < 0.001).

Condition	K1	A1	K2	A2
	min–1	%	min–1	%
d-Amph (100 nM)	0.027 ± 0.01	22 ± 6	0.19 ± 0.02	88 ± 5
d-Amph (100 nM) + SoRI-9804 (0.4 μM)†	0.028 ± 0.013	33 ± 18	0.14 ± 0.04	74 ± 16
d-Amph (100 nM) + SoRI-9804 (2.0 μM)†	0.035 ± 0.01	59 ± 12*	0.21 ± 0.07	50 ± 10*
d-Amph (100 nM) + SoRI-9804 (10 μM)†	0.021 ± 0.004	58 ± 11*	0.15 ± 0.05	45 ± 10*
d-Amph (100 nM) + SoRI-9804 (25 μM)†	0.023 ± 0.004	65 ± 10*	0.19 ± 0.06	43 ± 9*

^*The A values of the curve (constraint 4; see Materials and Methods) were significantly different (F-test, P < 0.01) from the 100 nM d-Amph curve. The kinetic constants of each curve did not significantly differ from those of the 100 nM d-Amph curve

^†The entire curve (constraint 1; see Materials and Methods) was significantly different (F-test, P < 0.01) from the 100 nM d-Amph curve