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. 2009 Feb 6;329(2):505–514. doi: 10.1124/jpet.108.147934

Fig. 8.

Fig. 8.

Parthenolide induces promoter hypomethylation methylation-silenced genes and their re-expression. A, perturbation of HIN-1 promoter methylation in MCF-7 cells exposed to indicated concentrations of parthenolide. DNA (1 μg) from parthenolide-treated or untreated MCF-7 cells were treated with bisulfite followed by PCR amplification, M.SssI methylation, and digestion to nucleosides. 5mdC and 2dC in the hydrolysates were measured using the LC-MS/MS method, and the ratio of 5mdC to 2dC is used as a DNA methylation indicator. B, hypomethylation activities of parthenolide (10 and 30 μM) and decitabine (0.75 μM) on HIN-1 promoter detected by bisulfite-sequencing. A 15% decrease of the promoter methylation in parthenolide-treated group was found. The solid circles represent methylated CpG loci, and the open circles represent nonmethylated CpG loci. C, parthenolide increased expression of HIN-1 gene in MCF-7 cells treated with parthenolide for the indicated time points and dosages. HIN-1 gene expression was measured by real-time RT-PCR. Conc, untreated; DAC, decitabine (0.75 μM); Par, parthenolide.