Fig. 3.

Effects of flumazenil on carisoprodol activity at GABA_A receptors. A, representative traces demonstrating carisoprodol-mediated currents and the potentiation of GABA-gated currents by diazepam and carisoprodol in the presence and absence of flumazenil. B, coapplication of diazepam potentiated GABA-gated currents to 238.3 ± 28.7% of control values. The actions of diazepam were blocked by a saturating concentration of flumazenil. Coapplication with carisoprodol potentiated GABA-gated currents to 210.7 ± 14.9% of control values. The allosteric actions of carisoprodol were not significantly different in the presence of flumazenil (218.6 ± 17.1% of control). Likewise, carisoprodol-mediated currents were 113.8 ± 4.9% of control values in the presence of flumazenil (N.S., p > 0.05). Each bar represents the mean ± S.E. of data collected from four cells.