Fig. 3.

SFKs are involved in SP-induced STAT3, NFκB, and AP-1 activation in pancreatic acinar cells. Freshly isolated pancreatic acini were preincubated with PP2 at 1 and 10 μM or vehicle (dimethyl sulfoxide) followed by stimulation with 1 μM SP for 45 min. The cells were separated from incubation medium by centrifugation. The pellet (cells) was used for STAT3 (a), NFκB (b), and AP-1 (c) nuclear extraction. STAT3, NFκB, and AP-1 DNA-binding assays were then carried out as described under Materials and Methods. The results are representative of three independent (n = 3) experiments. Results shown are the means + SE. ^*, P ≤ 0.05 compared with control; +, P ≤ 0.05 compared with SP.