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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Gastroenterology. 2008 Dec 9;136(3):1081–1090. doi: 10.1053/j.gastro.2008.12.006

Figure 7. Identification of miR-30a targets in BMEL cells.

Figure 7

(A) Confirmation of microarray results by quantitative RT-PCR of RNA from transfected BMEL cells. Genes identified in the microarray analysis as significantly altered by α30a transfection were selected. Candidate gene expression was normalized to Hprt expression, and the results are expressed relative to a value of 1 in the control ASO-treated cells. (B) Luciferase reporter assays to test for regulation of candidate gene 3’-UTRs by miR-30a. The indicated 3’-UTRs were sub-cloned into a dual luciferase reporter plasmid. Luciferase activity was normalized for both transfection efficiency and for effects on a reporter plasmid lacking the 3’-UTR. The results are expressed relative to a value of 1 in the control ASO-treated cells. *p < 0.05