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Role of global and virulence regulators in production of curli. EPEC E2348/69 and sogenic mutants were monitored for production of curli fibers by ELISA using anti-curli antibodies. Pre-immune antiserum was used as negative control. The experiments were performed three times in triplicate on separate days with samples collected from T-medium after overnight growth and the results represent the mean of the averages obtained from those three experiments.
