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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Environ Microbiol. 2009 Nov 14;11(4):992–1006. doi: 10.1111/j.1462-2920.2008.01824.x

Fig. 6.

Fig. 6

Fis is a negative regulator of curli. (A) and (B) Electron microscopy micrographs showing lack of curli in E2348/69 (A) and presence of curli in E2348/69Δfis (B) after growth in LB broth. (C) Detection of curli by flow cytometry and (D) immunoblotting of E2348/69, E2348/69Δfis and E2348/69Δfis(pFis) using anti-curli antibody. (E) Kinetics of curli expression in E2348/69 and E2348/69Δfis, between 1–9 h of growth in LB as determined by immunoblotting. Detection of DnaK was used as a loading control.