Table 1.
E. coli strains and plasmids used in this study.
| Strains | Notes | Source |
|---|---|---|
| EDL933 | EHEC (O157:H7) Wild type | (Riley et al., 1983) |
| EDL933ΔcsgA | csgA::km | This study |
| EDL933ΔbcsA | bcsA::cm | This study |
| EDL933ΔcsgD | csgD::km | This study |
| EDL933ΔcsgAΔbcsA | Double csgA::km/bcsA::cm mutant | This study |
| E2348/69 | EPEC (O127:H6) Wild type | (Levine et al., 1978) |
| E2348/69ΔcsgA | csgA::km | This study |
| E2348/69ΔbcsA | bcsA::cm | This study |
| E2348/69ΔcsgD | csgD::km | This study |
| E2348/69ΔfisΔcsgD | Double fis::cm/csgD::km mutant | This study |
| E2348/69ΔcsgAΔbcsA | Double csgA::km/bcsA::cm mutant | This study |
| E2348/69Δfis | fis::km | This study |
| E2348/69(pMLB1034) | Promoter-less control | This study |
| E2348/69ΔgrlA | grlA::km mutant | Bustamante et al., unpublished |
| E2348/69ΔgrlR | grlR::km mutant | Bustamante et al., unpublished |
| E2348/69ΔgrlRA | grlRA::km mutant | Bustamante et al., unpublished |
| E2348/69Δler | ler::km mutant | Bustamante et al., unpublished |
| E2348/69Δhns | hns::km mutant | Bustamante et al., unpublished |
| E2348/69Δihf | Δ himA::km mutant | Bustamante et al., unpublished |
| E2348/69ΔperA | Δ perA::km mutant | Bustamante et al., unpublished |
| E2348/69ΔperC | ΔperC::km mutant | Bustamante et al., unpublished |
| E2348/69ΔqseA | ΔqseA::km mutant | This study |
| Plasmids | ||
| pBAD-Topo | Cloning vector | Invitrogen |
| pCsgA | E2348/69csgBA in pBAD-Topo | This study |
| pBcsA | E2348/69bcsA in pUC19 | This study |
| pFis | E2348/69fis in pUC19 | This study |
| pCP994 | csgD in pKK233-2 | (Prigent-Combaret et al., 1999) |
| pMLB1034 | Promoter-less lacZ | (Uhlich et al., 2002) |
| pW5lac | csgD::lacZ in pMLB1034 | (Uhlich et al., 2002) |
Single csgA, bcsA, csgD and fis mutants were complemented with pCsgA, pBcsA, pCP994 or pFis, respectively.