Fig 6.

Effect of siRNA on herpes simplex virus 1 (HSV-1). Vero cells were transfected with 50 pmoles of siRNA1 directed against sybl1. 24 hrs after transfection, cells were infected with HSV-1 at an MOI of 1. After 60 min. at 37°C, the inoculum was removed and fresh medium was added. Cells and supernatants were harvested 48 hrs after infection, and virus titres were determined by plaque assay. Titres are expressed as percentages of the virus yield, where 100% represents the titre of virus grown in wells transfected with control siRNA. Values are means ± SD from three independent experiments. The asterisk indicates a statistically significant difference with P= 0.0007 (Student's t-test).