Table 2. Proteins of HS serum granulations and nanobacteria identified by MALDI-TOF mass spectrometry.

#	Identified Proteinsa	MWb (kDa)	Serum Granulationsc				Nanobacteriad
			HS-1	HS-2	HS-3	HS-4	HS NB
1	Complement component 3	187	8	10	8	15	12
2	Serum albumin	69	11	8	5	6	14
3	Apolipoprotein B100	516	8	6	11	8	6
4	Complement component 4A	193	7	4	4	7	6
5	Apolipoprotein A1	31	2	8	6	5	4
6	α2-macroglobulin	163	3	4	5	1	10
7	IGHM protein	53	3	2	2	1	2
8	Serotransferrin	77	3	0	0	0	4
9	Apolipoprotein A2	11	1	2	1	1	1
10	Haptoglobin	47	1	2	2	0	0
11	Fetuin-A	39	0	1	1	1	1
12	Hemoglobin subunit β	16	1	0	0	0	0

^aThe numbers shown for each protein correspond to the amount of distinct peptides obtained for each protein which was divided by the total amount of distinct peptides identified in the first 50 proteins found in each sample. This value was then multiplied by 100 to give an indication of the relative abundance of each protein.

^bMW: molecular weight.

^cSerum granulations (pellets) were obtained from untreated HS (HS-1) or following addition of either CaCl₂ (HS-2), Na₂HPO₄ (HS-3), or both (HS-4) as described in the Materials and Methods .

^dHS NB was obtained from human serum as described in the Materials and Methods .