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. Author manuscript; available in PMC: 2009 Apr 24.
Published in final edited form as: Cell Cycle. 2004 Jul 4;3(7):960–967. doi: 10.4161/cc.3.7.1003

Figure 2.

Figure 2

FEAR mutant slk19 is defective in anaphase concentration of condensin. (A) Condensin-to-nucleolus concentration in the synchronous cell cycle. Parallel analysis of 640-BY4741 (WT) and 640-4002451-BY4741 (slk19-Δ) strains released from alpha-factor arrest allowed comparative monitoring of Smc4p-GFP dynamics in the nucleus. Diffuse condensin, light green nucleus; nucleolus-targeted condensin, crescent-shaped dark-green subnuclear structure. (B) Micrograph of the 90-min point in the time-course analysis of the slk19-Δ strain. Arrow 1, unique class of cells undergoing anaphase with mistargeted condensin; Arrow 2, mid-to-late anaphase recapture of condensin by nucleolus. Scale bar 5 μm.