Skip to main content
. Author manuscript; available in PMC: 2009 Apr 24.
Published in final edited form as: Nat Chem Biol. 2008 May 30;4(7):418–424. doi: 10.1038/nchembio.94

Figure 6.

Figure 6

Effect of leucascandrolide analog 3 and neopeltolide 2 on cellular respiration, NADH (28) consumption and activity of cytochrome bc1 complex. Data represent one typical experiment. (a) Effect of oligomycin A (26) on respiration of 143B cells. The initial inhibition of respiration was restored by the addition of proton uncoupler CCCP (27). (b) Inhibition of cellular respiration of 143B cells by leucascandrolide A analog 3. Subsequent treatment of cells with CCCP (27) did not restore respiration. (c) Effect of taxol (25, 2.5 μM), rotenone (29, 2.5 μM), and leucascandrolide A analog 3 (0.5 μM) on NADH level in HCT116 cells before and after treatment (bar graphs on the left and the right, respectively). (d) Dose-dependent inhibition of purified bovine cytochrome bc1 complex by analog 3 and neopeltolide 2.