Figure 1.
Binding of anti-fHbp mAbs to purified recombinant fHbp as measured by ELISA. The proteins were expressed from genes from strains MC58 (variant 1), 8047 (variant 2) and M1239 (variant 3), respectively. A, Binding of mAb JAR 4 to antigenic variant 1 and 2 proteins but not with a variant 3 protein. B, Binding of a control mAb, anti-fHbp, JAR 5, which recognizes a variant 1-specific epitope in the N-domain involving amino acid residues 121 and 122 (Beernink et al., 2008).