Table 2.
Amino acid sequences of the phage-displayed peptides mimicking the JAR 4 epitope
| Clonea | Sequenceb | Number of clones | ELISA ODc |
|---|---|---|---|
| 1 | H D H K L E G T E | 5 | |
| 2 | C G G V Y D D K T G C A | 3 | 2.0–3.5 |
| 3 | H D H K T Q L D P | 1 | |
| 4 | W T L A V F D H K A Q T | 2 | 1.0–1.9 |
| 5 | G C M G Y D H R S G C V | 1 | |
| 6 | F H D H K T A N Q | 1 | 0.5–0.9 |
| 7 | H D H R I W P L D V T A | 1 |
a
The positive phage clones, ranked by their reactivity with JAR 4.
b
Deduced amino acid sequences of the peptide inserts displayed through pVIII fusion on the phage library clones positive for JAR 4 binding. The most conserved residues are indicated in bold.
c
mAb JAR 4 reactivity of isolated clones, determined by ELISA.