Skip to main content
. Author manuscript; available in PMC: 2010 Feb 18.
Published in final edited form as: Vaccine. 2009 Jan 13;27(8):1154–1165. doi: 10.1016/j.vaccine.2008.12.035

Figure 5. Sera background binding is an intrinsic property of IgG.

Figure 5

A. Specific response, following background subtraction, to a subset of recombinant vaccinia antigens on the micro-ELISA array using sera from a vaccinated individual (1/300). B. Following Cibacron Blue (F3GA)-agarose treatment of the sera to remove albumin, specific responses with background subtracted are also reduced indicating partial removal of the IgG component. C. Following pretreatment of the sera with Protein A-agarose removing the IgG component, all specific anti-vaccinia protein reactivity is removed. D. For this individual, the background independent of any antigen presence was high; removal of albumin reduced the background by almost 0.5, but reduced the IgG signal by a similar amount (see panel B). Removal of the IgG component alone eliminated the background entirely.