TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| Strain | ||
| P. aeruginosa | ||
| PAO1 | Serotype O5, type strain | (27) |
| E. coli | ||
| BL21(DE3) | General expression strain F-ompT hsdSB(rB-mB-) gal dcm (DE3) | Novagen |
| Rosetta(DE3) | Expression strain with pRARE plasmid (CmR) encoding rare tRNAs | Novagen |
| Tuner(DE3) | Expression strain with lacY deletion for strict regulation of induction by IPTG | Novagen |
| Plasmid | ||
| pLysS | Expresses T7 lysozyme to suppress basal expression of T7 RNA polymerase prior to induction | Novagen |
| pET-28a | 5.4-kb T7 expression vector with N- or C-terminal His tags, KmR | Novagen |
| pET-23dr | Derivative of pET-23a T7 expression vector with short linker between N-terminal His tag and protein, AmpR | (18) |
| pCQW14 | PCR from PAO1 template inserted NdeI-XhoI into pET-28a to generate His6-WbpA expression plasmid, KanR | (9) |
| pET-His6-WbpB | PCR from PAO1 template inserted BamHI-EcoRI into pET-28a to generate His6-WbpB expression plasmid, KanR | (7) |
| pWMJL085 | PCR from PAO1 template DNA inserted NcoI-BamHI into pET-23dr vector to generate His6-WbpE expression plasmid, KanR | (7) |
| pCQW3 | PCR from PAO1 template DNA inserted NdeI-HindIII into pET-28a to generate His6-WbpD expression plasmid, KanR | (8) |
| pWMJL072 | pET-23dr-based His6-WbpI expression plasmid, AmpR | (11) |