FIGURE 3.

SLAT is recruited to the phagosome in FcγR-stimulated macrophages. A, bone marrow-derived macrophages from wild-type mice were stimulated with human IgG-coated latex beads. The cells were fixed, permeabilized, and stained with antibodies to SLAT or GAPDH as indicated; NRS and normal rabbit IgG (NRIg) were used as negative controls. All cells were stained with phalloidin to detect F-actin. Representative optical slice from a confocal micrograph of BMM after 3 min of ingestion of human IgG-coated latex beads, stained with the indicated antibodies and with phalloidin. B, representative four serial optical slices from a confocal micrograph showing recruitment of SLAT on the phagosome along with F-actin indicated with arrows. C, mean fluorescence intensity of SLAT and F-actin stain around 15 phagosomes. Data represent average from three independent experiments.