FIGURE 5.

Cdc55-dependent sur4 suppressor activity in rvs161 cells does not function through regulating actin polarization. Strains were grown in YEPD or selective minimal media at 30 °C to exponential phase, and at time zero, NaCl was added to a final concentration of 3.4% (A and B) or 6% (C). Actin structure was visualized at the indicated times using Rhodamine-phalloidin and fluorescence microscopy. Histogram showing the average percentage of polarized cells versus total number of cells was determined (n = 350). Error bars represent the standard deviation. D, cells lacking Rvs161 are viable on 3.4% NaCl. 10-fold serial dilutions of cells were spotted on YEPD, 3.4% NaCl, and 6% NaCl solid media. Cells were grown for 3 days at 30 °C.