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A, Fru-1,6-P2 titration curves. B, phenylalanine titration curves for PK-WT (•) and the K422R (○) and H391Y (▾) mutant proteins. Enzyme activity was assayed at 25 °C and pH 7.5 as described under “Experimental Procedures” with 2.5 mm P-enolpyruvate and 2.5 mm ADP.
