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The CD spectra of PK-WT and the mutant proteins were recorded in the far-UV region (from 200 to 250 nm) at 25 °C at a concentration of 0.3 mg/ml in 10 mm phosphate buffer (pH 7.5), 3 mm MgCl2, 100 mm KCl, and 5% glycerol. The mean residual ellipticity (MRE) was plotted for a wavelength from 200 to 250 nm. deg, degrees.
