TABLE 2.
Effect of activator Fru-1,6-P2 and inhibitor Phe on activity of PK-WT and mutant enzymes
|
Protein
|
Fru-1,6-P2 saturation
kinetics,aactivity
increasedb
|
Effect of
Phea
|
|||||
|---|---|---|---|---|---|---|---|
|
Activity
leftc
|
Ki
|
||||||
| -Fru-1,6-P2 | +Fru-1,6-P2 | -Fru-1,6-P2 | +Fru-1,6-P2 | ||||
| % | % | mm | |||||
| PK-WT | 20.5 ± 1 | 23.5 ± 1 | 40.7 ± 2 | 0.176 ± 0.02 | 0.261 ± 0.05 | ||
| K422R | 20 ± 1.5 | 20 ± 2 | 32.1 ± 3.1 | 0.067 ± 0.009 | 0.162 ± 0.04 | ||
| H391Y | 42.5 ± 3 | 76.5 ± 3.3 | 81.12 ± 4 | 1.431 ± 0.1 | 1.78 ± 0.07 | ||
a
Done at fixed concentrations of 2.5 mm P-enolpyruvate and 2.5 mm ADP.
b
Done at ≥2.5 mm Fru-1,6-P2.
c
Done at 1.0 mm Phe.