FIGURE 4.

Exogenously applied CaM alters the Ca²⁺ flux across the plasma membrane of protoplasts isolated from lily pollen grains. A, lily protoplast illustrating the location of the three scanning ion-selective electrodes (arrows) employed to simultaneously record the plasma membrane Ca²⁺ fluxes. B, affect of CaM or QD-CaM probe on net Ca²⁺ flux. Note that both CaM and QD-CaM caused a net influx of Ca²⁺ into lily protoplasts. The data represent the average values obtained for the three scanning ion-selective electrodes. The positive values represent Ca²⁺ efflux, whereas the negative values represent influx.