Summary of the EH truncation mutants and their interaction.
A, schematic of full-length E and H subunits and truncation mutants
used in this study. Secondary structure prediction was obtained from the
PSIPRED prediction server. The arrows, cylinders, and lines
denote predicted β-sheet, α-helix, and random coil content,
respectively. B, table of interaction assay results. Positive and
negative interactions are designated with (+ and -), indicating the
ability/inability to co-purify by either ion exchange or gel filtration.
Interactions not assessed in this study are marked
(···).