FIGURE 1.

Schematic outline of the RIP-Chip analysis to identify Msi1 associated mRNAs. 293T cells were transfected transiently with BAP-tagged constructs. BAP-tagged proteins were biotinylated in vivo by the co-transfected E. coli BirA enzyme. RNPs were recovered via precipitation with streptavidin-Sepharose beads. Finally, RNAs were purified and analyzed on microarrays. We used a dual approach to identify Msi1 preferentially associated mRNAs. Two different controls (BAP-GFP and BAP-PABP1) were used in two independent sets of experiments. The results of the two sets of experiments were compared leading to the identification of 64 Msi1-associated mRNAs.