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Rate of Ca2E1P → E2P. The decay of the Ca2E1P phosphoenzyme calculated as the difference between the total amount of phosphoenzyme and the ADP-insensitive E2P fraction (difference between closed and open circles in Fig. 3) was fitted by a monoexponential decay function as illustrated in supplemental Fig. S5, and the extracted rate constants are shown relative to that of the wild type (WT).
