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Mutant VCP alters endogenous TDP-43 localization 24 h post-transfection. At 24 h post-transfection, dsRED-VCP fusion protein (red), TDP-43 (green), and TOPRO-3 (nuclear marker; blue) were visualized by confocal microscopy (arrows indicate TDP-43 and VCP colocalization and accumulation (merge/yellow)).
