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Frog CgA expression induces the biogenesis of secretory granule-like structures in COS-7 cells. Immunogold electron microscopy of COS-7 cells transfected with pIRES-fCgA (A) or with the empty vector (B). Scale bars equal 1 μm. Insets show dense-core vesicles containing the protein (arrowheads) in fCgA-expressing cells and unlabeled organelles such as mitochondria in empty vector-transfected cells. Scale bars equal 100 nm. m, mitochondria; N, nucleus.
