FIGURE 7.

Deletion of the N- and/or C-terminal regions of fCgA markedly reduces its granulogenic activity in COS-7 cells. A, schemes depicting the different fCgA constructs used. B, COS-7 cells transfected with plasmids encoding full-length fCgA or fCgA-truncated forms were examined for fCgA immunoreactivity using confocal microscopy. Three-dimensional reconstructions of confocal stacks were used to quantify automatically the granules with a diameter > 200 nm. Values for the number of granules/cell, indicated under each representative image, are given as the means ± S.E. (n = 10;^**,p < 0.01; ^***p<0.001). Scalebars, 10 μm. C, COS-7 cells were transfected with plasmids encoding full-length fCgA or N- and C-terminally deleted protein, and examined for fCgA and GM130 immunoreactivities using confocal microscopy. Scale bars, 10 μm.