FIGURE 1.
Schematic representation of the bait and prey used in the yeast two-hybrid screen. A, schematic representation of full-length TrkA and the pGilda-TrkAIC bait. TrkA has a single transmembrane domain and a single cytoplasmic tyrosine kinase domain. C, cysteine clusters; LRR, leucine-rich repeats; Ig, immunoglobulin-like domain; TM, transmembrane domain; TK, tyrosine kinase domain. B, the two-hybrid screen identified the region of Mint2 containing the PTB domain (amino acids 286-539) as a binding partner for TrkAIC. N, N-terminal region; PDZ, the PDZ domain. aa, amino acids.