FIGURE 1.
Purification and characterization of UDP-GlcNAc pyrophosphorylase. A, purified AGX2 (1.5 μg) was subjected to SDS-PAGE on a 10% (w/v) acrylamide gel, as described under “Experimental Procedures.” Molecular masses (in kDa) are indicated on the left. B and C, the UDP-GlcNAc and PPi dependence of the AGX2-catalyzed synthesis of UTP was assessed in the presence of 0.1 unit/ml AGX2 and either 1 mm PPi (B) or 2 mm UDP-GlcNAc (C). The data represent the mean value ± S.D. of two independent experiments (n = 4).