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. 2009 Apr;126(4):535–542. doi: 10.1111/j.1365-2567.2008.02917.x

Figure 4.

Figure 4

The biological activity of four recombinant interleukin-32 (rIL-32) isoforms in human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated with four different rIL-32 isoforms (40 ng/ml) in the presence of polymyxin B (100 U/ml) for 18 hr, after which the cell culture supernatant was harvested for cytokine measurement. (a) IL-6 and (b) tumour necrosis factor-α (TNF-α), were measured by enhanced chemiluminescence. As shown in the second lane, lipopolysaccharide (LPS) induced a large amount of cytokine in both IL-6 and TNF-α, whereas polymyxin B (100 U/ml) in the third lane, abolished LPS activity.