Figure 5.
The examination of recombinant interleukin-32 (rIL-32) isoform protein with the peritoneal macrophages of lipopolysaccharide (LPS)-resistant C3H/HeNj mice. The peritoneal macrophages of normal C57-Black/6 and LPS-resistant C3H/HeNj mice were stimulated four rIL-32 isoforms and then mouse (a) macrophage inflammatory protein 2 (MIP-2) and (b) tumour necrosis factor-α (TNF-α) were measured in the cell culture supernatant after 18 hr of stimulation. (a) and (b), Lane 6, staphylococcus stimulates a normal response in both normal C57-Black/6 and LPS-resistant C3H/HeNj peritoneal macrophages, whereas LPS, lane 7, shows a lack of response in LPS-resistant C3H/HeNj peritoneal macrophages.