Table 1.
Primers and probes of real-time PCR for allelic discrimination of ABCC2 SNPs in Caucasians
| cDNA position1 | SNP | Exon | Amino acid change | Eense-/antisense primer | Probes2 |
| 1249 | G>A | 10 | V417I | 5’-CCAACTTGGCCAGGAAGGA-3’/ | VIC 5’-CTGTTTCTCCAACGGTGTA-3’ |
| 5’-GGCATCCACAGACATCAGGTT-3’ | FAM 5’-ACTGTTTCTCCAATGGTGTA-3’ | ||||
| 3563 | T>A | 25 | V1188E | 5’-GCACCAGCAGCGATTTCTG-3’/ | VIC 5’-ACACAATGAGGTGAGGAT-3’ |
| 5’-AGGTGATCCAGGAAAAGACACATTT-3’ | FAM 5’-ACAATGAGGAGAGGAT-3’ | ||||
| 4544 | G>A | 32 | C1515Y | 5’-GTAATGGTCCTAGACAACGGGAAG-3’/ | VIC 5’-AGAGTGCGGCAGCC-3’ |
| 5’-CCAGGGATTTGTAGCAGTTCTTCAG-3’ | FAM 5’-ATTATAGAGTACGGCAGCC-3’ |
1
cDNA sequence from GenBank accession numbers NM_000392 starting at the ATG;
2
For each SNP two probes were designed and labeled with the fluorescent reporter dyes VIC (allele 1) and FAM (allele 2). SNP: Single nucleotide polymorphism.