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. 2009 Feb 17;37(7):2116–2125. doi: 10.1093/nar/gkp057

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Expression and purification of E. coli KsgA. Escherichia coli KSR7 carrying pGEX-KsgA was induced by 0.4 mM of IPTG at 24°C for 8 h. Proteins were separated by 12% SDS–PAGE and stained with Coomassie Blue. Lanes 1 and 7, molecular weight marker; lane 2, soluble fraction disrupted by sonication; lane 3, flow-through fraction from glutathione-Sepharose column; lane 4, purified GST–KsgA fusion protein after elution from glutathione-Sepharose column; lane 5, fusion protein after digestion by thrombin; lane 6, purified KsgA after elution from Hi-Trap SP column.