Table 1.
Designation | Sequence | Enzyme tested |
---|---|---|
5-foU/N | 5′-GGTCGACTFAAGGTACC | KsgA, |
3′-CCAGCTGANTTCCATGG | MutY | |
Tg/N | 5′-GGACGACAXAAGGAACC | KsgA |
3′-CCTGCTGTNTTCCTTGG | ||
5-hmU/C | 5′-GATCCTCTAGAGHCGACCTGCA | KsgA |
3′-CTAGGAGATCACCGCTGGACGT | ||
8-oxoG/C | 5′-GAACTAGTG8ATCCCCCGGGCTGC | KsgA |
3′-CTTGATCACCTAGGGGGCCCGACG | ||
2-ohA/C | 5′-GAACTAGTG2ATCCCCCGGGGCTGC | KsgA |
3′-CTTGATCACCTAGGGGGCCCCGACG | ||
Tg/C# | 5′-CGGCGCGCXCGGGCGGC | KsgA |
3′-GCCGCCCGCGCGCGCCG | ||
Tg/A# | 5′-GCCGCCCGXGCGCGCCG | Nth, Nei |
3′-CGGCGGGCACGCGCGGC | ||
8-oxoG/A | 5′-GAACTAGTG8ATCCCCCGGGCTGC | MutY |
3′-CTTGATCACATAGGGGGCCCGACG |
F, X, H, 8 and 2 represent 5-foU, Tg, 5-hmU, 8-oxoG and 2-ohA, respectively.