Table 2.
Escherichia coli strains used for spontaneous mutation assay
| Strains | Relevant genotype | Construction |
|---|---|---|
| CSH101 | Wild-type | |
| CSH101m | mutM::Kan | |
| CSH101y | mutY::Tet | |
| CSH101my | mutM::Kan mutY::Tet | |
| CSH101k | ksgA::Kan | |
| CSH101c | car96::Tet | |
| CSH101kc | ksgA::Kan car96::Tet | P1(CSH101c) × CSH101k |
| CSH101yk | mutY::Tet ksgA::Kan | P1(CSH101y) × CSH101k |
| CSH101mkc | mutM::Kan ksgA::kan car96::Tet | P1(CSH101kc) × CSH101m |
| CSH101mkΔc | mutM::Kan ksgA::kan Δcar96::Tet | Deletion of car96::Tet in CSH101mkc |
| CSH101myk | mutM::Kan mutY::Tet ksgA::Kan | P1(CSH101y) × CSH101mkΔc |
| CSH101-3 | nth::Kan | |
| CSH101z | zdh925::Tet | |
| CSH101-3z | nth::Kan zdh925::Tet | P1(CSH101z) × CSH101-3 |
| CSH101-3zk | nth::Kan zdh925::Tet ksgA::Kan | P1(CSH101-3z) × CSH101k |
| CSH101-8 | nei::Cm | |
| CSH101-38k | nth::Kan nei::Cm ksgA::Kan | P1(CSH101-8) × CSH101-3zk |
The strains were constructed by P1 transduction. CC101mkΔc was isolated according to Bochner et al. (32).