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hGINS expression is linked to proliferation. (A) Abundance of hGINS in different cell lines. Immunoblot detection of Psf1 in whole cell extracts prepared from 50 000 cells of each cell line. The indicated amounts of recombinant GINS were used as reference. Levels of GAPDH are shown as loading control. (B) Immunoblot detection of hGINS subunits and the indicated proteins in asynchronously proliferating (A), quiescent (G0) and re-plated (R) BJ-hTERT fibroblasts.
