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. 2009 Feb 17;37(7):2070–2086. doi: 10.1093/nar/gkp067

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Knock-down of HMGB1 and HMGB2 results in inhibition of topo IIα expression. Nuclear lysates from untransfected (lanes 1) and mock-transfected (lanes 2) cells, as well as from cells transfected with plasmids producing shRNAs specific for HMGB1 (lanes 3, construct #A) or HMGB1/HMGB2 (lanes 4, construct #B) were resolved on SDS-polyacrylamide gels and transferred onto the membranes by western blotting. Proteins were detected by specific antibodies as detailed in the Materials and methods section.