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. 2009 Feb 17;37(7):2153–2163. doi: 10.1093/nar/gkp076

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Expanded contour plots of regions of a 500 MHz NOESY (300 ms mixing time) spectrum recorded at 25°C with the sample dissolved in phosphate buffer pH 5.9 (10 mM phosphate, 50 mM NaCl and 1 mM EDTA), depicting interactions of the exocyclic α-OH-PdG protons. Blue and red labels differentiate NOE peaks originated from the S and R enantiomers, respectively. Labels are assigned as follows: A, (S)X6(Hα)-(S)X6(Hγ/γ′); B, (S)X6(Hα)-(S)X6(Hβ/β′); C, (R)X6(Hα)-(R)X6(Hβ/β′); D, (S)X6(Hα)-C5(H2′); E, C5(H5)- (S)X6(Hγ/γ′); F, C5(H5)- (R)X6(Hγ); G, C5(H5)- (S)X6(Hβ/β′); H, (R)X6(Hγ)-(R)X6(Hγ′); I, (S)X6(Hγ)-(S)X6(Hγ′). The β and γ protons of α-OH-PdG were not stereo specifically assigned.