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. 2009 Feb 23;37(7):2264–2273. doi: 10.1093/nar/gkp085

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Mapping of the Tn599 insertions. (a) PFGE image of a set of clones (lanes 2–16) carrying the transposon, after digestion with PI-SceI. (b) Corresponding Southern blot hybridized with labeled probe. Data from (a) and (b) together allows the correct mapping of the transposon in each clone. Lanes 1 and 17 corresponds to Low Range PFGE Marker (NEB) and Lane 18 is Mid Range I PFGE Marker (NEB). Run conditions were 6 V/cm, 14°C for 20 h and a pulse time ramp of 0.4–10 s.