Figure 2.
Affinity of yeast heterotrimeric RPA complexes containing mutations in RPA2 for dT30. Binding constants of wild-type or mutant forms of RPA were determined by FP assays at 1.25 M KCl. The average association constant (Ka) for each mutant is presented. The experiments were performed at least three times with the standard deviation shown. *The only mutant that had an association constant that was statistically different than wild-type (using a one-way ANOVA with a Tukey post hoc analysis) was RPA•2(WF).