Figure 3.

Sensitivity of rfa2 mutant strains to UV. AMSY1 cells containing the indicated rfa2 gene on a plasmid were grown at 30°C to log phase (OD₆₀₀ ∼0.5) and the indicated number plated on YEPD plates. The plates were irradiated with the respective doses of UV light and incubated in the dark at 25°C for 3 days. Only a subset of the doses is shown. The rad52-1 strain (Bob Malone, University of Iowa) and rfa2-Δwh [deletion of the C-terminal winged-helix domain; (31)] were used as controls.