Table 1.

Nomenclature of mutants

Mutations in yeast RPA2	Abbreviation	Primers	Restriction site
S159A,N161A	SN	AAG CCC ATA GAT GCA TTC GCA GAA GTG TTG ACG CAT CAC	NsiI
		GTG ATG CGT CAA CAC TTC TGC GAA TGC ATC TAT GGG CTT
V134V,K135A,F137A	KF	GAA ATT GGC GGT TAC GTC GCA GTT GCA GGT GCT TTG AAA GAG	[SnaB1]
		CTC TTT CAA AGC ACC TGC AAC TGC GAC GTA ACC GCC AAT TTC
T79T,D80A,T82A	DT	GGT GTG GTG AGA AAC ATT ACT GCG CAT GCT GCA AAT ATT	FspI
		AAT ATT TGC AGC ATG CGC AGT AAT GTT TCT CAC CAC ACC
L96I,E97A,R99A,K100A	ERK	GA ACT GGT CAA TTG GCA GTG AGA AAA TGG AGC GAA GAT G	MfeI
		CAT CTT CGC TCC ATT TTC TCA CTG CCA ATT GAC CAG TTC
		GA ACT GGT CAA TTA GCA GTG GCA GCA TGG AGC GAA GAT G	[MfeI]
		CAT CTT CGC TCC ATG CTG CCA CTG CTA ATT GAC CAG TTC
G145G,K146A,K147A	KK	GCT TTG AAA GAG TTT GGT GGC GCC GCA AAT ATA CAG	KasI
		CTG TAT ATT TGC GGC GCC ACC AAA CTC TTT CAA AGC
D158A,E162A,V163V	DE	AAG CCC ATA GCT TCA TTC AAT GCA GTT TTG ACG CAT	[HincII]
		GAT GCG TCA AAA CTG CAT TGA ATG AAG CTA TGG GCT T
E103A,D104A	ED	GA AAA TGG AGC GCG GCC GCA AAT GAC TTG GC	EagI
		GCC AAG TCA TTT GCG GCC GCG CTC CAT TTT C
W101A,F143A,G144G	WF	GAA GTG AGA AAA GCT AGC GAA GAT GCA	NheI
		TGC ATC TTC GCT AGC TTT TCT CAC TTC
		GGT GCT TTG AAA GAG GCC GGC GGT AAG AAA AAT A	NgoMIV
		TAT TTT TCT TAC CGC CGG CCT CTT TCA AAG CAC C
T79T,D80A,T82A, L96I,E97A,R99A,K100A, G145G,K146A, K147A	MM1	same as for DT, ERK, KK	FspI, MfeI, KasI
T79T,D80A,T82A,D158A, E162A,V163V, E103A, D104A	MM2	same as for DT, DE, ED	FspI, EagI, NheI

Desired mutations shown in bold, conservative mutations used to aid cloning shown in plain text. Nucleotide changes in primers indicated in bold. Restriction site created or [eliminated] indicated in final column.